Investigation
- Routine Investigation: Haemoglobin estimation
- Blood counts: Selective rise in WBC (leucocytosis) seen in all infections and leukemias
- Low platelet count is seen in hypoplastic anaemia, leukemias.
- Thrombocytosis (increase in platelet count) is a feature of iron deficiency anaemia
- ESR: raised in case of anaemia except in sickle cell anaemia
- Peripheral Smear: Normocytic normochromic RBC is a feature of anaemia due to infections
- Macrocytic anaemia- vitamin B12 deficiency, folate deficiency, malabsorption
Special Investigations
Serum Iron and Iron Binding Capacity
- Used in differentiating pure iron deficiency from thalassemia. In iron deficiency anaemia, serum iron is decreased and iron-binding capacity is increased
- In thalassemia serum iron is raised and iron-binding capacity is decreased
Serum Ferritin Estimation
- It is decreased in iron deficiency and increased in anaemia of chronic inflammation
Urine urobilinogen
- Increased in haemolytic anaemia
Bone Marrow Examination
- In aplastic anaemia, leukemia
Radiology
- Chest X-ray- cardiomegaly in chronic anaemia
- Skull X-ray- hair on end appearance- congenital haemolytic anaemia
Karyotyping
- Fanconi’s anaemia- chromosomal breaks